Fig. 4: TRPM7 kinase domain is required for PANC-1 migration and invasion. | Cell Death & Disease

Fig. 4: TRPM7 kinase domain is required for PANC-1 migration and invasion.

From: The kinase domain of TRPM7 interacts with PAK1 and regulates pancreatic cancer cell epithelial-to-mesenchymal transition

Fig. 4

A Cell viability of PANC-1 Control and ΔK cells assessed by MTT assay during 96 h (Two-way ANOVA). B Cell migration of PANC-1 Control and ΔK cells assessed by wound-healing assay (n = 3, p < 0.001, Student-t test). Photographs were taken at magnification x50 right after the wound (T–0H) and 24 h after (T–24H). C Cell migration of PANC-1 Control and ΔK evaluated in Boyden chambers (n = 3, p < 0.001, Mann–Whitney test). D Cell migration of PANC-1 Control and ΔK treated with TG100-115 (50 µM) for 24 h (n = 3, p < 0.001, Two-way ANOVA followed by Šidák’s post-hoc test). E Cell invasion of PANC-1 Control and ΔK evaluated in Boyden chambers coated with Matrigel (n = 3, p < 0.001, Mann–Whitney test). F Cell invasion of PANC-1 Control and ΔK treated with TG100–115 (50 µM) for 24 h (n = 3, p < 0.001, Two-way ANOVA followed by Šidák’s post-hoc test). All the scale bars are corresponding to 200 µm. Results are shown as mean ± SD. ***p < 0.001.

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