Fig. 5: TRPM7 kinase domain interacts with PAK1. | Cell Death & Disease

Fig. 5: TRPM7 kinase domain interacts with PAK1.

From: The kinase domain of TRPM7 interacts with PAK1 and regulates pancreatic cancer cell epithelial-to-mesenchymal transition

Fig. 5

A Protein expression levels of pFAK and FAK in PANC-1 Control and ΔK cell lines (n = 6, p < 0.001, Two-way ANOVA followed by Šidák’s post-hoc test). B Protein expression levels of pPXN and PXN in PANC-1 Control and ΔK cell lines (n = 3, p < 0.05 and p < 0.01, Two-way ANOVA followed by Šidák’s post-hoc test). C Detection of focal adhesion complexes by immunofluorescence targeting pFAK, FAK and PXN. D Evaluation of the RhoGTPase family genes Pak1, Cdc42, Rac1, Rac2, Rac3 and RhoA assessed by RT-qPCR (n = 8 p < 0.05 and p < 0.01, Two-way ANOVA followed by Šidák’s post-hoc test) and by Western-Blot (n = 4, p < 0.05, p < 0.01 and p < 0.001, Two-way ANOVA followed by Šidák’s post-hoc test). E Co-immunoprecipitation of TRPM7 with PAK1 and RAC proteins in PANC-1 Control and ΔK cells and their respective positive and negative controls. F Proximity Ligation assay of TRPM7 with PAK1 in PANC-1 cells incubated with DMSO (n = 162) or 50 µM TG100-115 (n = 177) for 24 h (Mann–Whitney test). Scale bar = 20 µm. Results are shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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