Fig. 5: IFITM3 and ITGB3 regulate MMT in macrophages. | Cell Death & Disease

Fig. 5: IFITM3 and ITGB3 regulate MMT in macrophages.

From: Cellular senescence promotes macrophage-to-myofibroblast transition in chronic ischemic renal disease

Fig. 5

A Triple immunofluorescence analysis identified MMT cells co-expressing macrophage (CD68, red), senescence activation (p16INK-4a, green), and myofibroblast (α-SMA, pink) markers under different experimental conditions. Scale bar: 20 µm. B Semi-quantitative analysis of average fluorescence intensity for CD68, p16INK-4a, and α-SMA-positive cells demonstrated that silencing ITGB3 or IFITM3 using siRNA significantly reduced the colocalization of these markers. In contrast, treatment with an IFITM3 plasmid increased the immunofluorescent colocalization of CD68, p16INK-4a, and α-SMA, suggesting a role for IFITM3 in promoting MMT marker expression. Data are mean ± SD (n = 3/group). *P < 0.05 vs. Normal control (NC) and Non-SC groups; #P < 0.05 vs. SC group; and P < 0.05 vs. SC + IFITM3 over-expressing groups.

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