Fig. 5: The combination of NAMPT inhibitors and disulfiram decreases the total number of viable cells. | Cell Death & Disease

Fig. 5: The combination of NAMPT inhibitors and disulfiram decreases the total number of viable cells.

From: Targeting metabolic vulnerability by combining NAMPT inhibitors and disulfiram for treatment of recurrent ovarian cancer

Fig. 5

A Chemical structure of disulfiram. B Relative cell viability after treatment with disulfiram in 3D-cultured IGROV1 and A2780 cells for 72 h (n = 4 independent experiments). C The kinetic graph shows cell proliferation in 3D-cultured IGROV1 and A2780 cells with GNE-617, disulfiram, and combinatorial treatment at the indicated doses as a function of time (hours). The total number of viable cells with combinatorial treatment was set to 1 (n = 4 independent experiments). D Synergy plots generated by Combenefit showing the interaction between disulfiram and GNE-617 in 3D-cultured IGROV1 and A2780 cells. Analysis of the interaction resulted in HSA values (n = 4 independent experiments). E Left: Representative figures showing a change in ALDH activity using ALDEFLUOR fluorescence (FITC) in 3D-cultured IGROV1 cells after treatment with GNE-617, disulfiram, and combinatorial treatment at the indicated doses for 48 h. Cells included in the trapezoid represent ALDEFLUOR-positive cells. Right: Comparison of ALDH activity using geometric MFI in 3D-cultured IGROV1 as described above (n = 4 independent experiments). F Chemical structure of NCT-505. G Left: Representative images showing changes in ALDH activity using ALDEFLUOR fluorescence (FITC) in 3D-cultured IGROV1 cells after treatment with NCT-505 at the indicated doses for 48 h. Cells included in the trapezoid represent ALDEFLUOR-positive cells. Right: Comparison of ALDH activity using geometric MFI in 3D-cultured IGROV1 as described above (n = 3 independent experiments). H The kinetic graph shows cell proliferation in 2D-cultured IGROV1 and A2780 cells with NCT-505 at the indicated doses relative to Control over time (hours) (n = 4 independent experiments). I Synergy plots generated by Combenefit showing the interaction between NCT-505 and GNE-617 in 3D-cultured IGROV1 and A2780 cells. Analysis of the interaction resulted in HSA values (n = 4 independent experiments). Graph data were presented as mean ± SD of multiple experiments.

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