Fig. 1: Identification of double-positive cells with GBM and MSCs markers.

A t-SNE map displaying the clustering of 3459 cells. Each dot represents an individual cell, colored according to its cluster. B The CopyKat algorithm was used to distinguish between benign (diploid) and malignant (aneuploid) cells, presented in a t-SNE plot. C Bar plots illustrating the mRNA expression levels of the indicated markers, measured by log2-TPM (transcripts per million reads). Each bar represents a single cell, colored by the cell types identified in (A). D A t-SNE plot showing the distribution of double-positive (double-pos) cells, colored by cell types. E IF images showing the cell co-expressing GFAP (red), CD90 (orange), CD73 (yellow), and CD105 (green) in GBM specimens (GBM506). Scale bar: 20 μm (left), 5 μm (right).