Fig. 5: Hybrids have an enhanced ability to induce macrophage recruitment and M2 polarization. | Cell Death & Disease

Fig. 5: Hybrids have an enhanced ability to induce macrophage recruitment and M2 polarization.

From: Fusion of glioma-associated mesenchymal stem/stromal cells with glioma cells promotes macrophage recruitment and M2 polarization via m6A modification of CSF1

Fig. 5

A Representative images and statistical results of the macrophage transwell assay. Scale bar:50 μm. B Western blot analysis of CD206 expression in macrophages treated with CM supernatant from GBM cells or hybrids for 48 h. C FCM measurement of CD206 levels in THP-1-macrophages from each group. D Representative images and statistical results of IHC staining for CD68, CD206 and CD163 levels in the different groups. Scale bar: 20 μm. E Representative images and statistical results of IF staining showing the distribution and proportion of CD68 and CD206 positive cells in the different groups. Scale bar: 40 μm. F Flow cytometry analysis of the proportion of F4/80+ and F4/80+/CD206+ positive cells among CD11b+ cells in different groups. G Representative bioluminescence images and statistical results 3 weeks after cell implantation in mice treated with clodronate or PBS.

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