Fig. 7: FTO regulates CSF1 expression in an m6A-YTHDF2-dependent manner. | Cell Death & Disease

Fig. 7: FTO regulates CSF1 expression in an m6A-YTHDF2-dependent manner.

From: Fusion of glioma-associated mesenchymal stem/stromal cells with glioma cells promotes macrophage recruitment and M2 polarization via m6A modification of CSF1

Fig. 7

A IGV analysis illustrating the m6A modification levels of CSF1 mRNA in U87MG and Hybrid86 based on Me-RIP-seq data. B Schematic diagram of the predicted m6A modification site in CSF1 mRNA as identified by SRAMP. C Detection of m6A modification levels of CSF1 mRNA in GBM cells and hybrids using the MeRIP m6A Kit. D Detection of the m6A modification levels of CSF1 mRNA in control and FTO knockdown hybrids using the MeRIP m6A Kit. E, F qPCR analysis of CSF1 mRNA levels in control and FTO knockdown hybrids after actinomycin D treatment for 0, 1, 2, 3, and 4 h. G YTHDF2 was immunoprecipitated and then subjected to qPCR to assess CSF1 transcript levels. H qPCR analysis of CSF1 mRNA levels in hybrids with FTO knockdown alone and with both FTO and YTHDF2 knockdown. I qPCR analysis of CSF1 mRNA levels in hybrids with FTO knockdown alone and with both FTO and YTHDF2 knockdown after actinomycin D treatment for 0, 1, 2, 3, or 4 h.

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