Fig. 6: TC-I 15 inhibits OSCC and augments chemotherapy responsiveness of OSCC.

A The molecular formula of TC-I 15. B Co-IP analysis in CAL27 and HSC3 cells treated with DMSO and TC-I 15. C CAL27 cells were intracardially injected into mice. Six days later, TC-I 15 was injected (20 mg/kg) into mice via vein every third day. D Representative images showing the xenograft model in CA27 cells treated with DMSO or TC-I 15 (n = 5 per group) and the growth of tumor grafts was shown; data are presented as mean ± SD, statistical significance was determined using Student’s t-test. DMSO Dimethylsulfoxide. E Immunoblot assay of phosphor-ERK1/2, total-ERK1/2, phosphor-AKT, total-AKT, phosphor-YAP1, and total-YAP1 protein levels in xenograft tissue. p phosphor. F Representative FACS plots and quantification of CD133 + cells in the xenograft tissues; data are presented as mean ± SD from three independent experiments, statistical significance was determined using Student’s t-test. G CAL27 cells were intracardially injected into mice. Six days later, TC-I 15 (20 mg/kg) and Cisplatin (5 mg/kg) were injected into nude mice via vein every third day. H Representative images showing the xenograft model in CA27 cells treated with DMSO or TC-I 15 (n = 5 per group) and the growth of tumor grafts were shown; data are presented as mean ± SD, and statistical significance was determined using Student’s t-test.