Fig. 5: SFRP1 inhibits Wnt signaling and other stemness regulators. | Cell Death & Disease

Fig. 5: SFRP1 inhibits Wnt signaling and other stemness regulators.

From: Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation

Fig. 5

A Heatmap of differential gene expression in SFRP1-depleted keratinocytes. Differentially expressed genes (DEGs) between SFRP1-depleted and control keratinocytes were identified on day 0 and day 2 timepoints. DEGs were defined by threshold of p value ≤ 0.05 and | log2 (fold change)| > 1. B Top enriched GO terms (ranked by p-value) related to Wnt signaling. C Heatmap of gene expression for representative genes in (B). D Top enriched GO terms (ranked by p value) related to stem cell regulation. E Heatmap of gene expression for representative genes in (D). Genes associated with Wnt signaling are labeled in red. F Distribution of SFRP1-associated differential ATAC-seq peaks across genomic regions. Differential peaks were defined by threshold p value ≤ 0.05 and |fold change| > 1.5. G Top enriched Wnt GO (ranked by p value) of differential ATAC-seq associated genes. H Venn diagram of DEGs in RNA-seq and differential peak-associated genes in ATAC-seq. I Chromatin accessibility plot at the LIF genomic locus (chr22:30, 240, 453- 30, 246, 759). Shadowed fold change peaks meet threshold of >1.5 (shSFRP1 vs. shControl). Tracks were aligned with Ensembl LIF regulatory build and H3K27ac ChIP-seq data from foreskin keratinocytes.

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