Fig. 5: Y-27632 inactivates ROCK1 and interferes with cytoskeleton formation. | Cell Death & Disease

Fig. 5: Y-27632 inactivates ROCK1 and interferes with cytoskeleton formation.

From: Extracellular matrix stiffness in endometrial cancer: driving progression and modulating treatment sensitivity via the ROCK1/YAP1 axis

Fig. 5

A Immunofluorescence staining of the cell cytoskeleton with different levels of ROCK1 expression using phalloidin staining. B Growth inhibition curves of HEC-1A cells and Ishikawa cells treated with Y-27632 for 24, 48, and 72 h, respectively. C Expression of ROCK1 and related proteins after treatment with a time gradient of 10 µM Y-27632 in Ishikawa cells. D The impact of 0, 10, and 100 µM Y-27632 on HEC-1A and Ishikawa cell apoptosis was evaluated by flow cytometry. E The impact of 0, 10, and 100 µM Y-27632 on HEC-1A and Ishikawa cell cycle was evaluated by flow cytometry. F The impact of 0, 10, and 100 µM Y-27632 on HEC-1A and Ishikawa cell cytoskeleton was evaluated by immunofluorescence. Scale: 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, Error bar refers to mean and SD.

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