Fig. 4: Expression of ECM-related components in CCCs.
From: Digoxin promotes anoikis of circulating cancer cells by targeting Na+/K+-ATPase α3-isoform
A Surface expression levels of α3NaK and α1NaK in detached MKN45 cells under the presence (+) and absence (−) of ECM components (fibronectin, collagen, and laminin; 15 μg/ml). Representative Western blot images using antibodies against α3NaK (110 kDa) and α1NaK (100 kDa) in the total lysates (input) and biotinylation samples (surface) were shown in the upper panels. In the lower panels, surface expression level of α3NaK and α1NaK was quantified. n = 4. **p < 0.01. B Western blotting was performed using anti-integrin α5 (140 kDa) or fibronectin (260 kDa) antibody in gastric cancer tissues. C Immunocytochemistry was conducted with antibodies against integrin α5 and fibronectin in gastric cancer tissues. Scale bars, 10 μm. Immunocytochemistry was performed using antibodies against EpCAM (red) and integrin α5 (D; green) or fibronectin (E; green) in CCCs from gastric patients. Scale bars, 10 μm.
