Fig. 8: Mechanism study of B4 induced mitotic catastrophe. | Cell Death & Disease

Fig. 8: Mechanism study of B4 induced mitotic catastrophe.

From: Pharmacological inhibition of PLK1/PRC1 triggers mitotic catastrophe and sensitizes lung cancers to chemotherapy

Fig. 8

A Co-immunoprecipitation was conducted to examine the interaction between PLK1 and PRC1 in A549/DDP cells after B4 treatment. B Immunostaining was performed to show the localization of PLK1 and PRC1 in A549/DDP cells after B4 treatment at 48 h. Arrows indicate multinucleated cells. Images shown are representatives of three independent experiments. Expression of some cell cycle-related proteins after B4 treatment in A549/DDP cells. Whole cell proteins were detected by western blotting (C) using indicated antibodies and quantified with imageJ (D). Each has the expression of GAPDH as the internal control. E, F Expression of some cell cycle-related proteins after B4 treatment in A549 cells. Whole cell proteins were detected by western blotting (E) using indicated antibodies and quantified with imageJ (F). Data are presented as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001.

Back to article page