Fig. 6: Serine metabolism promotes M2 macrophage polarization through SAM paracrine signaling. | Cell Death & Disease

Fig. 6: Serine metabolism promotes M2 macrophage polarization through SAM paracrine signaling.

From: Chemotherapy-induced macrophage CXCL7 expression drives tumor chemoresistance via the STAT1/PHGDH-serine metabolism axis and SAM paracrine feedback to M2 polarization

Fig. 6

A Expression levels of IRF4 and STAT6 in macrophages treated with conditioned medium derived from indicated tumor cells. B Flow cytometry analysis of M2 macrophage marker expression in macrophages treated with the indicated conditioned medium. C Immunofluorescence staining showing CD206 expression in macrophages treated with the indicated conditioned medium (scale bar, 100 μm). D CXCL7 mRNA expression levels in macrophages exposed to conditioned medium from cancer cells treated with a PHGDH inhibitor, subjected to SAM rescue. E IHC staining of CRC samples showing the correlation between CXCL7 expression and PHGDH, p-STAT1, and CD206. Representative images of low and high CXCL7 expression cases are displayed, along with the percentage distribution of CRC samples exhibiting low or high CXCL7 expression relative to PHGDH, p-STAT1, and CD206 levels (scale bar, 100 μm). F Schematic representation of the correlation between CXCL7 expression and serine metabolism-related pathways in CRC, highlighting its role in M2 macrophage polarization and a potential feedback loop within the tumor microenvironment. All data are presented as mean ± SD. *P < 0.05; n.s.not significant.

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