Fig. 5: FAT10-NCOA4 complex is not degraded by the proteasome pathway. | Cell Death & Disease

Fig. 5: FAT10-NCOA4 complex is not degraded by the proteasome pathway.

From: Mechanistic insights into the role of FAT10 in modulating NCOA4-mediated ferroptosis in pancreatic acinar cells during acute pancreatitis

Fig. 5

A HA-FAT10-p62 and HA-FAT10-NCOA4 fusion plasmids were transfected into FAT10-/- HEK293T cells at different doses. The expression of HA-FAT10-p62 and HA-FAT10-NCOA4 complexes was observed with or without MG132 (15 μM); B GST- pull-down analysis for FAT10 and RPN10 in AR42J cells; C Colocalisation of FAT10 and RPN10 in AR42J cells (Scale bar: 10 μm); D, E FAT10-/- HEK293T cells were transfected with HA-FAT10-p62 and HA-FAT10-NCOA4 plasmids combined with His-Rpn10 or shRpn10 plasmids and were then exposed to cycloheximide (CHX, 20 μM). Western blotting analysis was performed for the indicated time and quantified by ImageJ software. ***p < 0.001. ns not significant.

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