Fig. 6: Silibinin inhibits the FAT10-NCOA4 axis to reduce ferroptosis in vitro and in vivo. | Cell Death & Disease

Fig. 6: Silibinin inhibits the FAT10-NCOA4 axis to reduce ferroptosis in vitro and in vivo.

From: Mechanistic insights into the role of FAT10 in modulating NCOA4-mediated ferroptosis in pancreatic acinar cells during acute pancreatitis

Fig. 6

A The experimental schedule of cerulein-induced AP with saline and Silibinin pretreatment (n = 6 each group); B–D Colorimetric analysis of serum concentration of amylase, lipase and tissue concentration of trypsin; E, F Colorimetric analysis of tissue concentration of TNF-α and IFN-γ; G Gross appearance of pancreas; H The pancreatic injury was determined by HE staining. (Scale bar: 100 μm, 20 μm) (black arrows: acinar cell death; blue arrows: acinar vacuolisation; yellow arrows: infiltration of inflammatory cells; green arrows: pancreatic interstitial oedema); I Western blotting analysis of FAT10, NCOA4, FTH1, TFRC and ACSL4 expression in DMSO and Silibinin-treated AR42J cells; J Colorimetric analysis of Fe2+ levels in DMSO and Silibinin-treated AR42J cells; K Quantification of mean C11 fluoresence intensity of DMSO and Silibinin-treated AR42J cells stained with C11-BODIPY; L Western blotting analysis of FAT10, NCOA4, FTH1, TFRC and ACSL4 expression in the pancreas of rats; M Representative immunohistochemical staining of FAT10, NCOA4, FTH1, TFRC and ACSL4 in the pancreas of rats. (Scale bar: 100 μm, 20 μm); N Colorimetric analysis of Fe2+ levels and Perls Prussian blue staining of pancrea slices (Scale bar: 20 μm); O Measurement of MDA levels and MDA staining of pancrea slices (Scale bar: 50 μm). *p < 0.05; **p < 0.01; ***p < 0.001.

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