Fig. 3: MAPK1 is a druggable gene whose expression is clinically relevant in multiple pan-cancer datasets.
From: Genes driving three-dimensional growth of immortalized cells and cancer
a The distribution of the 85 3D exclusive dropout genes in each of the top 20 molecular pathways from Fig. 2c shown as a Matrix plot, where each purple dot represents involvement of each gene in the molecular pathways. The 24 genes shown in yellow are involved in ≥9 of the 20 molecular pathways. The red font genes are the ones which were not involved in the top 20 molecular pathways, and hence excluded from this current study. b Forest plot of the 24 genes shows the hazard ratio (HR), as well as the p value, illustrating the overall survival of BLCA patients. The blue dotted line in the middle of the plot shows an HR of 1. The 11 genes with an HR > 1 and a p value < 0.05 have the p value highlighted in red with red asterisks and written in red fonts. Genes with HR < 1 but p value < 0.05 are marked with black asterisks and written with black fonts. c Bar graph showing normalized MAPK1 gene expression across the cell transformation stages among the patient pool in GSE3167 dataset. d The expression of MAPK1 and DCLK1 in the TCGA BLCA cohort were the only two genes to show an increase among the patients who had higher stages of BLCA. e The 11 genes were assessed in the pan-cancer MetMap 500 dataset which assess the metastatic potential of cell lines, and only MAPK1 and ROCK1 showed a positive correlation with metastatic potential. f In the MetMap 500 dataset concerning exclusively BLCA cell lines, MAPK1 shows a positive correlation with a negative slope, consistent with the pan cancer analysis. The correlation is represented by a blue dotted line, which is the best fitted regression line. g In the MetMap 500 dataset concerning exclusively BLCA lines, ROCK1 does not show a positive correlation between gene effect and metastatic potential, which means that depleting ROCK1 gene in the BLCA cell lines increases the metastatic potential of the cell lines. h Radar plot shows the common metastatic sites for these BLCA cell lines, where they are likely to metastasize. i The expression level of MAPK1 in cell lines is plotted against the proliferation signature score for each cell line, with green regression line showing a positive correlation between increased MAPK1 expression and increased proliferation.
