Fig. 2: Multi-omics analyses identify exosomal CCT6A as a critical contributor to TAM M2 polarization. | Cell Death & Disease

Fig. 2: Multi-omics analyses identify exosomal CCT6A as a critical contributor to TAM M2 polarization.

From: Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma

Fig. 2

A Schematic diagram of experimental design assessing the potential PDAC-derived M2 TAM-induced exosomal proteins using proteomic detection and public datasets, which identifying CCT6A, CAP1, CAPZA1 and RHOA as the promising contributors. Created with BioRender.com. B IHC analysis of CCT6A and IF staining of CD68 (green) and CD163 (red) in tumor tissue sections from 33 PDAC patients. Scale bar, 100 μm. C Scatter diagram of the tumoral CCT6A expression levels and M2 macrophage infiltration scores in 33 PDAC patients. Simple linear regression and the Pearson correlation coefficient analysis. D Size distribution of human PDAC-derived exosomal particles, as measured by NTA. Blue represents exosomes derived from serum, and red represents exosomes derived from tumor. E Analysis of the expression of specific exosomal biomarkers and CCT6A in exosomes isolated from serum (left) and tumor (right) via western blotting. F Immunoelectron microscopy analysis of CCT6A in exosomes isolated from serum (left) and tumor (right). Scale bar, 50 nm. G Comparison of serum-originated exosomal CCT6A levels by western blotting. ‘P’ represents exosomes derived from serum of PDAC patients, while ‘N’ represents exosomes derived from serum of healthy individuals. (n = 5). n.s. no significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Back to article page