Fig. 2: NUSAP1 promotes proliferation and tumor growth in 5-FU-resistant gastric cancer cells.
From: PRMT1-catalyzed NUSAP1 methylation enhances Notch2 signaling and 5-FU resistance in gastric cancer
A Western blot analysis showing NUSAP1 protein levels in BGC-823-5-FU-R and SGC-7901-5-FU-R cells transfected with shNUSAP1 (shNUSAP1-1# and shNUSAP1-2#) or shGFP control. Tubulin served as a loading control. B Cell viability assay performed over 7 days using BGC-823-5-FU-R and SGC-7901-5-FU-R cells transfected with shNUSAP1 or shGFP control. ***p < 0.001. C, D EdU incorporation assay showing reduced proliferation of BGC-823-5-FU-R and SGC-7901-5-FU-R cells upon NUSAP1 knockdown. Representative EdU-stained images are shown in (C), and the percentage of EdU-positive cells is quantified in (D). Scale bar = 20 μm. ***p < 0.001. E, F Transwell migration assay performed to evaluate the migratory potential of BGC-823-5-FU-R and SGC-7901-5-FU-R cells transfected with shNUSAP1 or shGFP. Representative images are shown in (E), and the percentage of migrated cells is quantified in (F). Scale bar = 100 μm. ***p < 0.001.G, H Transwell invasion assay showing the invasive potential of BGC-823-5-FU-R and SGC-7901-5-FU-R cells transfected with shNUSAP1 or shGFP. Representative images are shown in (G), and the percentage of invading cells is quantified in (H). Scale bar = 100 μm. ***p < 0.001. I Soft agar assay was conducted to assess the self-renewal ability of NUSAP1-knockdown cells compared to shGFP control. Scale bar = 100 μm. J, K In vivo subcutaneous xenograft assay in nude mice injected with NUSAP1-knockdown or control BGC-823-5-FU-R and SGC-7901-5-FU-R cells. Representative tumor images are shown in (J), and tumor weights are quantified in (K). **p < 0.01. ***p < 0.001.
