Fig. 2: PHGDH inhibition or knockdown reduces the expression of proinflammatory cytokine in astrocytes. | Cell Death & Disease

Fig. 2: PHGDH inhibition or knockdown reduces the expression of proinflammatory cytokine in astrocytes.

From: PHGDH-mediated serine synthesis in astrocytes supports neuroinflammation by sustaining NADH level to promote histone acetylation

Fig. 2

A qRT-PCR analysis of TNF-α, IL-1β, and IL-6 mRNA expression in astrocytes treated with CNT-503 or DMSO followed by LPS stimulation for 4 h, n = 6. B ELISAs analysis of TNF-α, IL-1β and IL-6 levels in the supernatants from the cultures of astrocyte-treated with DMSO or NCT-503 followed by LPS treatment of LPS for 8 h, n = 3. C qRT-PCR analysis of TNF-α, IL-1β and IL-6 mRNA expression in astrocytes treated with CBR-5884 or DMSO followed by treatment with LPS for 4 h, n = 3. D Representative immunoblotting of siPHGDH or siNC transfected astrocytes. E qRT-PCR analysis of TNF-α, IL-1β, and IL-6 mRNA expression in siPHGDH or siNC transfected astrocytes followed by LPS stimulation for 4 h, n = 3. The data are means ± SD, for all panels: *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test (A–C, E).

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