Fig. 3: PHGDH fails to affect inflammatory signaling transduction in astrocytes. | Cell Death & Disease

Fig. 3: PHGDH fails to affect inflammatory signaling transduction in astrocytes.

From: PHGDH-mediated serine synthesis in astrocytes supports neuroinflammation by sustaining NADH level to promote histone acetylation

Fig. 3

A Representative images of PHGDH, GFAP and DAPI immunofluorescence staining in primary astrocytes treated with LPS or PBS. Scale bar, 20 μm, n = 3. B Representative immunoblotting of phosphorylation (p-) or total protein of astrocyte lysates treated with DMSO or NCT-503 in the presence or absence of LPS, n = 3. C Representative images of GFAP, P-P65 and DAPI immunofluorescence staining after astrocytes treated with DMSO or NCT-503 and treated PBS or LPS for 1 h. Scale bar, 10 μm, n = 3. The data are means ± SD, for all panels: n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test (B).

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