Fig. 3: CTSE affects DCP levels by regulating the production of vitamin K cycle. | Cell Death & Disease

Fig. 3: CTSE affects DCP levels by regulating the production of vitamin K cycle.

From: CTSE inhibits anti-tumor T cell response by promoting des-γ-carboxy prothrombin releasing in hepatocellular carcinoma

Fig. 3

A UMAP analysis reveals heterogeneity in PanCK+ cells associated with CTSE expression levels. B Volcano plot Showing differentially expressed genes between CTSE high and low expression patients based on PanCK analysis. C GSEA plots of ubiquinone signaling pathway in patients with CTSE high and low expression patients based on PanCK analysis. D Ubiquinone signaling pathway associated genes. Western blot was used to detect GGCX and NOX2 protein expression in Huh7 (E, F) and HepG2 cells (G, H) after CTSE knockdown and overexpression. I, J Western blot was used to detect CTSE, GGCX and NOX2 protein expression with or without pepstatin A (15 μM or 15 μM, 24 h) treatment. K, L Histogram of statistical analysis of DCP expression levels in Huh7 cell line after CTSE knockdown and overexpression. M, N Histogram of statistical analysis of DCP expression levels in Huh7 cell line culture medium after CTSE knockdown and overexpression. O Schematic representation of the role of CTSE in the Vitamin K cycle and its downstream effects. Date are resented are mean ± SEM. The p-values are calculated by student’s t test or one-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001.

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