Fig. 5: RNF41 regulates NUDC-induced tubulin depolymerization. | Cell Death & Disease

Fig. 5: RNF41 regulates NUDC-induced tubulin depolymerization.

From: Loss of RNF41 promotes bladder cancer metastasis through increasing NUDC stability to enhance tubulin polymerization

Fig. 5

Sequencing peaks of RNF41 (B) and NUDC (A) after RNF41 knockdown. C Gene Ontology (GO) analysis of differentially expressed genes following RNF41 knockdown in T24 cells. CC represents cellular component; MF denotes molecular function. D Western blot analysis was performed to assess protein levels in T24 and BIU cells stably expressing RNF41 shRNA. E Western blot was conducted on T24 and BIU cells stably expressing NUDC shRNA to evaluate the indicated protein levels. F T24 and BIU cells stably expressing either NUDC or RNF41 shRNA plasmids, with or without NUDC shRNA, underwent western blot analysis to measure specific protein levels. G T24 and BIU cells with stable RNF41 shRNA expression were treated with or without Monomethyl auristatin E (MMAE) (5 μM) and subsequently analyzed by western blot for the specified proteins. H Confocal microscopy images of T24 and BIU cells stably expressing Control or RNF41 shRNA. I Confocal microscopy images of T24 and BIU cells stably expressing Control or NUDC shRNA. J Confocal images of T24 and BIU cells stably expressing Control, RNF41 shRNA, and/or NUDC shRNA. K Confocal images of T24 and BIU cells stably expressing Control, RNF41 shRNA, and/or MMAE. Tubulin (green) was visualized using β-tubulin antibodies, while DNA (blue) was stained with DAPI. Scale bar, 20 μm.

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