Fig. 2: 643943 triggers CASP7-mediated apoptotic signaling by disrupting the XIAP:CASP7 complex. | Cell Death & Disease

Fig. 2: 643943 triggers CASP7-mediated apoptotic signaling by disrupting the XIAP:CASP7 complex.

From: Blocking XIAP:CASP7-p19 selectively induces apoptosis of CASP3/DR malignancies by a novel reversible small molecule

Fig. 2

A Immunoprecipitation/Western blot analysis of XIAP:CASP7 complex in MCF-7 cells and XIAP:CASP3 complex in MCF-10A treated with 643943 (20 μM) for 4 h, respectively. 643943 efficiently blocked the PPI between XIAP and CASP7. GAPDH was used as a loading control. B Cytocidal effects of 643943 on MCF-7 cells based on MTT assay. Cells were treated with 643943 at the indicated concentrations for 24 h and live cells were determined by MTT assay. C Cytocidal effects of 643943 on MCF-7 cells based on sub-G0 arrest. Cells were treated with 643943 at the indicated concentrations for 24 h. Accumulation of cells in the sub-G0 fraction was determined by PI-based flow cytometric analysis. D Cytocidal effects of normal MCF-10A cells. Cells were treated with 643943 at the indicated concentrations for 24 h. Accumulation of cells in the sub-G0 fraction was determined by PI-based flow cytometric analysis. E Determination of intracellular caspase activities in untreated (white bars) or 643943-treated (black bars) MCF-7 cells for 24 h. F Relative apoptosis percentages of MCF-7 cells treated with 20 μM 643943 for 24 h in the absence or presence of the CASP7 inhibitor (MPS) at the indicated doses. G Immunoblotting for CASP7 and GAPDH (control for protein loading) and H relative apoptotic percentages of the MCF-7 cells and the cells transfected with control shRNA (Con-shRNA) or specific shRNA (CASP7-shRNA). A–H Data were obtained from three independent experiments.

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