Fig. 3: HDAC3 plays a crucial role in regulating the homeostasis of mitochondrial oxidative stress in BMSCs. | Cell Death & Disease

Fig. 3: HDAC3 plays a crucial role in regulating the homeostasis of mitochondrial oxidative stress in BMSCs.

From: HDAC3-YY1-RAB5A axis remodels AML-supportive niche by modulating mitochondrial homeostasis in bone marrow stromal cells

Fig. 3

A PCA analysis plot. B Expression heatmap of differentially expressed genes. C volcano plot of all detected genes. D GSEA result showed inflammatory response gene set was significantly enriched both in shHDAC3 sequencing and GSE107490. E GO cellular component enrichment (GO_CC) analysis of genes downregluated in shHDAC3 group. F Schematic diagram of experimental flow. G, H Flow cytometry analysis of ROS level in HS-5 treated by Ctrl, HL60 CM, HL60 CM + BG45 after 72 h. The right panel is the statistics of mean fluorescence intensity. I, J Flow cytometry analysis of JC-1 fluorescence in HS-5 treated by Ctrl, HL60 CM, HL60 CM + BG45 after 72 h. K mRNA level of IL6, IL1B and HDAC3 in HS-5 cells induced by AML cells derived CM and CM + NAC. Data are presented as the mean ± SD. n = 3, *p < 0.05, **p < 0.01, **p < 0.001.

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