Fig. 5: HDAC3 inhibits YY1 acetylation to modulate RAB5A. | Cell Death & Disease

Fig. 5: HDAC3 inhibits YY1 acetylation to modulate RAB5A.

From: HDAC3-YY1-RAB5A axis remodels AML-supportive niche by modulating mitochondrial homeostasis in bone marrow stromal cells

Fig. 5

A UCSC, JASPAR and GEPIA2 databases were used to search for transcription factors that regulate RAB5A. B Candidate transcription factors interacting with HDAC3 were identified based on the STRING database (https://cn.string-db.org/). C HDAC3-flag was pulled down using anti-Flag antibody, and YY1 in the precipitated proteins was detected by immunoblotting. D Relative protein expression level of YY1 and RAB5A in HS-5 cells following treatment with varying concentrations of BG45. E Quantification of D. F Immunoblots of YY1 and RAB5A after knocking down HDAC3. G Immunoblots of RAB5A after YY1 overexpression. H Changes in the level of RAB5A mRNA after overexpressing YY1. I Immunoblots of RAB5A after knocking down YY1. J Immunoblots of RAB5A after complementation with YY1-Flag. K Immunoblots of YY1 expression after knocking down RAB5A. L Relative enrichment of YY1 at RAB5A promoter assessed by ChIP-qPCR. YY1 was pulled down using YY1 antibody, and the acetylation (M) and ubiquitination level (N) of YY1 after HDAC3 knockdown was detected by immunoblotting. All blots were normalized with a loading control. Data are presented as the mean ± SD. n = 3, *p < 0.05, **p < 0.01.

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