Fig. 2: TNF-α induces SOX4 expression and activates the TGF-β/Smad signaling pathway and its downstream genes.
From: TNF-α modulates cell proliferation via SOX4/TGF-β/Smad signaling in benign prostatic hyperplasia
A RT-PCR was used to test the mRNA levels of SOX4 in WPMY-1 and BPH-1 cells treated with TNF-α. B WB was used to test the protein levels of SOX4 in WPMY-1 and BPH-1 cells treated with TNF-α. C GEO data showing SOX4 expression in BPH samples from GSE119195, GSE132714, and GSE167196. D Representative SOX4 IHC staining and quantification in normal and BPH prostate samples. E, F WB analysis of TGF-β/Smad signaling protein expression in BPH-1 and WPMY-1 cells treated with TNF-α (5 ng/mL and 10 ng/mL) for 3 days. G, H RT-PCR and WB analysis of the mRNA and protein expression of EMT markers in BPH-1 cells treated with TNF-α (5 ng/mL and 10 ng/mL) for 3 days. I, J RT-PCR and WB analysis of the mRNA and protein expression of fibrosis markers in WPMY-1 cells treated with TNF-α (5 and 10 ng/ml) for 3 days. Data are expressed as the means ± SEMs (*p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant).
