Fig. 4: Overexpression of SOX4 regulates cell viability and cell cycle in BPH-1 and WPMY-1 Cells.
From: TNF-α modulates cell proliferation via SOX4/TGF-β/Smad signaling in benign prostatic hyperplasia
A–C RT-PCR and WB were used to confirm the SOX4 overexpression effect in BPH-1 and WPMY-1 cells infected with vector or SOX4 lentivirus for 3 days. D BPH-1 and WPMY-1 cells were plated in 96-well plates (3 × 103 cells/well) and infected with vector or SOX4 lentivirus for 0–3 days. Cell proliferation viability was determined by CCK-8 test, respectively. E, F Flow cytometry was used to test the cells cycle of BPH-1 and WPMY-1 cells infected with vector or SOX4 lentivirus for 3 days. Data are expressed as the means ± SEMs (*p < 0.05, **p < 0.01, ***p < 0.001, ns not significant).
