Fig. 7: Overexpression of SOX4 partially reverses the effects of Met-induced inhibition of BPH cell proliferation and TGF-β/Smad signaling activation.
From: TNF-α modulates cell proliferation via SOX4/TGF-β/Smad signaling in benign prostatic hyperplasia
A WPMY-1 cells were plated in 96-well plates (3 × 103 cells/well) overnight. Then, WPMY-1 cells were treated with Vector, Vector combined with Met, overexpression SOX4, or overexpression SOX4 combined with Met. for different time points (0, 24, 48, and 72 h). Cells viability was determined by CCK-8 test. B, C WPMY-1 cells were plated in 6-well plates (2 × 105 cells/well) overnight. Then, WPMY-1 cells were treated with Vector, Vector combined with Met, overexpression SOX4, or overexpression SOX4 combined with Met for 3 days, and harvested for cell cycle test via flow cytometry. D WPMY-1 cells were plated in 6-well plates (2 × 105 cells/well) overnight. Then, WPMY-1 cells were treated with Vector, Vector combined with Met, overexpression SOX4, or overexpression SOX4 combined with Met for 3 days, and harvested for cell apoptosis test via flow cytometry. Data are expressed as the means ± SEMs (*p < 0.05, **p < 0.01, ***p < 0.001).
