Fig. 3: ALKBH5 mediates MC-LR exposure-induced altered energy metabolism and cell proliferation inhibition. | Cell Death & Disease

Fig. 3: ALKBH5 mediates MC-LR exposure-induced altered energy metabolism and cell proliferation inhibition.

From: The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR

Fig. 3

Intracellular ATP levels (A) and extracellular lactate production (B) in liver tissue of mice exposed to MC-LR compared to vehicle control mice. The effect of ALKBH5 knockdown on intracellular ATP levels (C), extracellular lactate production (D), glucose uptake (E) and NAD+ /NADH ratios (F) was measured compared to control cells. G, H Effects of knockdown ALKBH5 on the colony-forming abilities of THLE-3 cells. I, J Effect of overexpression of ALKBH5 on colony forming ability of THLE-3 cells. Effects of ALKBH5 on intracellular ATP levels (K), extracellular lactate production (L), glucose uptake (M) and NAD+ /NADH ratios (N) of THLE-3 cells following MC-LR exposure. Effect of ALKBH5 overexpression (O and P) or knockdown (Q and R) on the colony forming ability of THLE-3 cells after exposure to MC-LR. Data are means ± SD from three independent experiments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.

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