Fig. 4: MC-LR exposure inhibits PIK3R1 expression mediated by ALKBH5. | Cell Death & Disease

Fig. 4: MC-LR exposure inhibits PIK3R1 expression mediated by ALKBH5.

From: The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR

Fig. 4Fig. 4

A Cell proliferation-related genes in liver tissue of mice exposed to MC-LR in RNA-seq data analysis. B Heatmap of RNA expression of the cell proliferation-related genes. C RT-PCR measurements of the related genes in liver tissue from MC-LR exposed mice. ALKBH5 knockdown reduced PIK3R1 mRNA (D) and protein (E, F) expression. Overexpression of ALKBH5 increased PIK3R1 mRNA (G) and protein (H, I) expression. MC-LR exposure resulted in the downregulation of PIK3R1 mRNA (J) and protein (K, L) expression, which was rescued by overexpression of ALKBH5. M Representative images of immunohistochemistry (IHC) analysis of ALKBH5 and PIK3R1 in liver tissue from MC-LR exposed mice. N IHC staining scores of ALKBH5 and PIK3R1 in liver tissue of mice exposed to MC-LR. Data are means ± SD from three independent experiments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

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