Fig. 7: Depresses of ALKBH5 suppresses mitochondrial oxidative phosphorylation during MC-LR exposure. | Cell Death & Disease

Fig. 7: Depresses of ALKBH5 suppresses mitochondrial oxidative phosphorylation during MC-LR exposure.

From: The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR

Fig. 7

A Cellular ROS levels determined in THLE-3 cells cultured under MC-LR exposure for 0, 6, 12, 24, 48 h. B Effects of overexpression of ALKBH5 on Cellular ROS levels exposed to MC-LR. C Analysis of mitochondrial oxidative phosphorylation enzyme genes in liver tissue of mouse exposed to MC-LR in RNA-seq data analysis. D Heatmap of RNA expression of the mitochondrial oxidative phosphorylation enzyme genes. E Specific regulation of ETC complex I activity by overexpression of ALKBH5 under MC-LR exposure. F, G The effect of MC-LR exposure on the expression of ETFDH, ETFA and NDUFAF4 in mouse liver tissue, as detected by Western blotting and quantified. Effect of ALKBH5 knockdown on ETFDH, ETFA and NDUFAF4 RNA (H–J) and protein (K, L) expression in THLE-3 cells. Data are means ± SD from three independent experiments. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.

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