Fig. 1: Excessive adipose tissue accumulation and PIK3CA gain-of-function of ADSCs in macrodactyly. | Cell Death & Disease

Fig. 1: Excessive adipose tissue accumulation and PIK3CA gain-of-function of ADSCs in macrodactyly.

From: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly

Fig. 1

A Gross appearance and radiograph showed diffuse tissue hyperplasia in macrodactyly. B Oil Red O and Masson’s trichrome staining revealing diffuse enlargement of adipocytes and localized collagen deposition of adipose tissue in macrodactyly compared with polydactyly. C Flow cytometric analysis of ADSC-specific markers (CD29, CD34, CD45, CD90, CD105, CD106) in Mac-ADSCs and Pol-ADSCs. D Sanger sequencing identifying PIK3CA mutations in Mac-ADSCs but not in Pol-ADSCs. E Western blot showing activation of the PI3K-AKT pathway in Mac-ADSCs. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 by unpaired t test.

Back to article page