Fig. 4: Knockdown of PIK3CA attenuates the paracrine effects of Mac-ADSCs. | Cell Death & Disease

Fig. 4: Knockdown of PIK3CA attenuates the paracrine effects of Mac-ADSCs.

From: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly

Fig. 4

(A) mRNA and (B) protein expression levels of PIK3CA in Mac-ADSCs after shRNA transfection. C Proliferation of ADSCs, FBs, and VECs when cocultured with shRNA-transfected Mac-ADSCs. D–F Migration and invasion capacities of (D) ADSCs, (E) FBs, and (F) VECs when cocultured with shRNA-transfected Mac-ADSCs. G, H Adipogenic differentiation potential of wild-type ADSCs evaluated by (G) mRNA expression levels of adipogenic markers and (H) Oil Red O staining after coculturing with shRNA-transfected Mac-ADSCs. I Tube formation capacity of VECs when cocultured with shRNA-transfected Mac-ADSCs. J–L Activation status of the PI3K-AKT signaling pathway in (J) ADSCs, (K) FBs, and (L) VECs when cocultured with shRNA-transfected Mac-ADSCs. Scale bars: 100 μm. Data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by ANOVA analysis.

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