Fig. 5: BYL-719 inhibits the paracrine effects of Mac-ADSCs. | Cell Death & Disease

Fig. 5: BYL-719 inhibits the paracrine effects of Mac-ADSCs.

From: Activating PIK3CA mutations in adipose-derived stem cells drive mutant-like phenotypes of wild-type cells in macrodactyly

Fig. 5

A Dose-response curve used to estimate the IC50 of BYL-719 in Mac-ADSCs. B Effect of BYL-719-treated Mac-ADSCs on proliferation of wild-type ADSCs, FBs, VECs. C–E Effect of BYL-719-treated Mac-ADSCs on migration and invasion capacities of (C) ADSCs, (D) FBs, and (E) VECs. F, G Adipogenic potential of wild-type ADSCs assessed by (F) mRNA levels of adipogenic markers and (G) Oil Red O staining after coculture with BYL719-treated Mac-ADSCs. H Tube formation capacity of VECs when cocultured with BYL-719-treated Mac-ADSCs. Scale bars: 100 μm. Data are presented as mean ± SD from three independent experiments. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by unpaired t test.

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