Fig. 7: Inhibition of USP14 with IU1-47 enhances radiotherapeutic abscopal effect via inducing ferroptosis. | Cell Death & Disease

Fig. 7: Inhibition of USP14 with IU1-47 enhances radiotherapeutic abscopal effect via inducing ferroptosis.

From: Targeting the TRIM14/USP14 axis enhances radiotherapy efficacy by inducing GPX4 degradation and disrupting ferroptotic defense in HCC

Fig. 7

Evaluation of the impact based on RT of tumor masses: A Schematic illustration. B Assessment of IU1-47 or (and) DFO on the secretion levels of IFN-β1 (B-1), CXCL10 (B-2), ISG15 (B-3), and IFN-γ (B-4) in the specified treatment groups of murine tumor masses. Results are presented as mean ± SD (n = 3). C Schematic representation of the experimental setup demonstrating the abscopal effect of RT on tumor-bearing mice (H22 tumors implanted bilaterally in the axillae) subjected to RT and IU1-47 or DFO. D Tumor growth trajectories for irradiated (IR) and non-irradiated (non-IR) mice administered IU1-47 or (and) DFO alongside vehicle (NS normal saline), divided into the 8 groups (n = 6/group) denoted in the figure. D-1, Irradiated tumor site; D-2, Abscopal tumor site. Left: tumor images; Right: tumor growth curves for each group. E Quantification of serum levels of IFN-β1, IFN-γ, and CXCL10 across the 8 groups. F Kaplan–Meier survival analyses of treated mice, illustrating survival outcomes across the aforementioned 8 groups (n = 6/group). Statistical significance was assessed via two-way ANOVA (n.s. not significant; ***, P < 0.001).

Back to article page