Fig. 1: Histopathology associated with disease onset in juvenile D2-mdx hearts. | Cell Death & Disease

Fig. 1: Histopathology associated with disease onset in juvenile D2-mdx hearts.

From: Failure to resolve inflammation contributes to juvenile onset cardiac damage in a mouse model of Duchenne muscular dystrophy

Fig. 1

Images show hearts harvested from juvenile (6 ± 0.5 wk) D2-mdx and B10-mdx mice at disease onset. A, B Whole tissue images with matched orientation of D2-mdx (A) and B10-mdx (B) hearts showing ventricular and atrial fibro-calcified damage. C, D Cross-sectional images of juvenile D2-mdx and B10-mdx hearts through the ventricular lumen, stained for histological features by H&E (C), and for fibrosis by Sirius Red (D). Arrowheads mark areas of fibrosis. E, F Image (E) and quantification (F) showing a portion of heart cross-section from juvenile D2-mdx and B10-mdx hearts, showing damaged tissue areas characterized by the presence of interstitial fibrosis, inflammatory cells and damaged cardiomyocytes. G, H Image (G) and quantification (H) showing a zoomed-in portion of heart cross-section from juvenile D2-mdx and B10-mdx hearts labeled with Sirius Red to mark fibrotic tissue area in hearts from juvenile D2-mdx and B10-mdx mice. Data represent median ± IQR from n = 10-12 hearts per cohort, with statistical analyses performed using non-parametric Mann–Whitney test; **p < 0.01, ***p < 0.001. Refer to Supplementary Figs. 1, 2 for additional details.

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