Fig. 1: PANC754 markedly inhibited the cell growth and metastasis of CRC cell lines. | Cell Death & Disease

Fig. 1: PANC754 markedly inhibited the cell growth and metastasis of CRC cell lines.

From: M6A-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer

Fig. 1

A Manhattan map of PANC754 crosses 23 types of human cancers. Cancer samples were collected from the TCGA project (N = 10,490). Gene expression level was log2 transformed before the meta-analysis. Random effect models were applied for the aggregation. B The expression levels of PANC754 were validated in the tumor tissues and paratumorous tissues of 26 pairs of colorectal cancer patients by quantitative PCR. C The proliferative curve of SW480 cell line with overexpression of PANC754 by CCK-8 assay. Control, untransfected SW480; pcDNA3.1, empty pcDNA3.1 plasmid transfected; PANC754, overexpression plasmid of PANC754 transfected; *P < 0.05. D Cell apoptosis detection by flow cytometry. Each experiment was repeated at least three times. E–G Determination of cell migration in DLD cell line by wound healing repair assay and its statistical histograms in 24 h F or 48 h. G ns, no significance; ***P < 0.001. H, I Cell invasion detection by transwell chamber assay and their statistical histograms in SW480 cell line. ****P < 0.0001. J–L MMP9 and EMT marker E-cadherin were detected by WB and their statistical histograms in the SW480 cell line. Each experiment was repeated at least three times.

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