Fig. 4: PANC754 was the nuclear-located and bound with its RBP PSPC1. | Cell Death & Disease

Fig. 4: PANC754 was the nuclear-located and bound with its RBP PSPC1.

From: M6A-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer

Fig. 4

A The subcellular location of PANC754 was detected by RNA FISH. U6 served as a nucleus location control; 18S served as a cytoplasm location biomarker. B The subcellular location of PANC754 by the nuclear-cytoplasmic fractionation experiment. GAPDH served as a cytoplasmic location biomarker. C The flowchart of RNA pulldown of PANC754 and subsequent analysis (Created with Microsoft PPT, and some icons in the schematic are sourced from bersinbio.com). D The agarose gel electrophoresis map verified that in vitro transcription approach to produce both sense and antisense RNA transcripts of PANC754. E The silver staining pattern of RNA pulldown between sense and antisense RNA transcripts of PANC754. F The Venn diagram of LS-MS/MS analysis between the sense and antisense RNA transcripts of PANC754. G Immunoblotting of the RNA pulldown samples further confirmed PSPC1 as the RBP for PANC754. H The mRNA level of PSPC1 in various CRC cell lines. I The mRNA level of PSPC1 when overexpression of PANC754 and/or knockdown of PSPC1. NC, HCT116 cells with untransfected plasmid; OE-PANC754, overexpression PANC754 plasmid transfected; shPSPC1, shPSPC1 plasmid transfected.

Back to article page