Fig. 5: PANC754 binding with its RBP PSPC1 suppresses CRC progress via inhibiting immune evasive molecule LGALS7. | Cell Death & Disease

Fig. 5: PANC754 binding with its RBP PSPC1 suppresses CRC progress via inhibiting immune evasive molecule LGALS7.

From: M6A-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer

Fig. 5

A The heat map of PANC754 overexpression in DLD1 cell line. NC, HCT116 cells with untransfected plasmid; 754, overexpression PANC754 plasmid transfected. B The volcano plot of PANC754 overexpression, where the arrow pointed LGALS7 significantly downregulated. C GSEA (gene set enrichment analysis) indicated that PANC754 overexpression led to the enrichment of the immune signals of leukocyte acitvation. D The mRNA levels of PANC754, PSPC1, and LGALS7 gene after PANC754 overexpression in DLD1 cells were detected by qRT-PCR. *P < 0.05; **P < 0.01; ***P < 0.001. Each experiment was repeated at least three times. E The mRNA levels of METTL3, PANC754, PSPC1, and LGALS7 gene after METTL3 overexpression in HCT16 cells were detected by qRT-PCR. F The mRNA levels of METTL3, PANC754, PSPC1, and LGALS7 gene after METTL3 knockdown in SW480 cells were detected by qRT-PCR. G The protein expression of PSPC1 and LGALS7 was determinedin SW480 cells by Western blotting. GAPDH served as the loading control; Empty vector, transfected with empty pcDNA3.1 plasmid group; OE-PANC754, transfected with pcDNA3.1-PANC754 overexpression plasmid group; shPANC754, transfected with shPANC754 kncokdown plasmid group.

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