Fig. 6: PANC754/PSPC1/H3K4me1 repression complex regulates LGALS7 expression.

A The protein-protein interaction (PPI) diagram from RNA-Seq data suggests a strong correlation between PANC754’s downstream effects and the histone-encoding gene H3-4. B, C The histone H3K4me1 protein level after PANC754 overexpression in SW480 cells was detected WB and their statistic histograms. C PCNA was served a internal control. ***P < 0.001. Each experiment was repeated at least three times. D The interplay between PSPC1 and H3K4me1 by molecular docking experiment. E The direct interaction between PSPC1 and H3K4me1 was detected in Caco2 cells by Co-IP. F By using H3K4 inhibitor MTA, the mRNA level of LGALS7 gene decreasing slowly company with the increasing concentration of MTA in DLD1 cells. G A schematic diagram showed the model of m⁶A-dependent nuclear ncRNA PANC754 coupled with its binding protein PSPC1 and chromatin-accessible H3K4me1 protein to form ncRNA/RBP/histone repression complex to downregulate the immune invasive LGALS7 signaling, inhibiting colorectal cancer progress (Created with Microsoft Visio).