Table 1 Immunocytochemical staining imaging settings.

From: Endothelial sensitivity to pro-fibrotic signals links systemic exposure to pulmonary fibrosis

 

Excitation max [nm]

Emission wavelength [nm]

Exposure [ms]

Gain [V]

Hoechst

348

455

450

662

VE-cad

577

603

670

681

a-SMA

493

517

540

641

ZO-1

577

603

670

681

No primary control

493

517

540

641

No primary control

577

603

670

681

  1. nm nanometres, ms milliseconds, V volts.