Fig. 3: NEDD4-2 substrates are elevated in db/db kidneys.
From: Nedd4-2 ablation in kidney improves glycaemic control in diabetic mice
A Immunofluorescence of 12-week-old male kidneys. B Male whole kidney lysates, ENaC band indicated by arrow quantitated relative to β-actin in (C), n = 3 mice. D Immunofluorescence of 12-week-old male kidneys. E Male whole kidney lysates, NCC quantitated relative to β-actin in (F), n = 3. G qPCR for NCC in 12-week-old kidneys, n = 5. Data presented as mean ± SD for immunoblots (C, F) or mean ± SEM for qPCR (G) and analysed by one-way ANOVA. *P < 0.05, **P < 0.01, ****P < 0.001.
