Fig. 5: HDV-mediated IFN-β production independently activates metabolic factors ATF4 and mTORC1. | Cell Death & Disease

Fig. 5: HDV-mediated IFN-β production independently activates metabolic factors ATF4 and mTORC1.

From: IFN-β production promotes metabolic rewiring and protection against oxidative stress in hepatitis delta virus-infected hepatocyte cultures

Fig. 5

A Relative mRNA (top panel) and protein (bottom panel) levels in HepaRGNTCP cells treated or not with 1 μM rux for the last 4 days of infection. Normalized to non-treated mock cells, n = 3. The Actin panel of the immunoblot is also used in Fig. S2E. B Relative mRNA levels in shScrambled (Scr) and shATF4 HepaRGNTCP cells infected or not with HDV. Normalized to scrambled mock cells, n = 5. C Relative mRNA (left panel) and protein (right panel) levels in HepaRGNTCP cells treated or not with 200 nM of torin 1 for the last 24 h of infection. Normalized to non-treated mock cells, n = 3. The non-treated panel of the immunoblot is also used in Fig. S1C and Fig. 2A. Statistical analysis: data are shown as mean +/− SD. For A–C Mann-Whitney or t-test or one-way ANOVA or one-way ANOVA on ranks with Tukey multiple comparison test were used. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.

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