Fig. 6: HDV infection renders cells resistant to oxidants and reduces functionality. | Cell Death & Disease

Fig. 6: HDV infection renders cells resistant to oxidants and reduces functionality.

From: IFN-β production promotes metabolic rewiring and protection against oxidative stress in hepatitis delta virus-infected hepatocyte cultures

Fig. 6

A Trypan blue staining of mock and HDV-infected HepaRGNTCP cells treated or not with 200 μM tBHP for 24 hours; 1 μM rux for 4 days; 10 μM BSO for 29 hours. B HDV/mock ratio of total glutathione (GSH) levels. HDV-infected HepaRGNTCP cells pre-treated or not with 10 μM BSO for 5 hours, and then treated or not with 200 μM tBHP for 2 hours. Normalized to non-treated mock cells, n = 4. C Relative albumin secretion (n = 6); CYP3A4 activity (n = 3); CYP1A2 activity (n = 3) in HepaRGNTCP cells infected with HDV. Normalized to mock cells. Statistical analysis: for B and C data are shown as mean +/− SD. For B and Albumin Mann-Whitney test was used. For CYP3A4 and CYP1A2 t-test was used. ***p < 0.001; **p < 0.01; *p < 0.05.

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