Fig. 3: Impact of METTL3/CXCL1 Signaling Axis on HPI GES-1 Cells. | Cell Death & Disease

Fig. 3: Impact of METTL3/CXCL1 Signaling Axis on HPI GES-1 Cells.

From: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

Fig. 3

A Colony formation assay to assess cell viability in HPI GES-1 cells. B Flow cytometry analysis to measure cell apoptosis in HPI GES-1 cells. C Western blot analysis of cleaved caspase-3, cleaved caspase-9, and cleaved PARP levels. D mRNA levels of IL-6, IL-8, and TNF-α in HPI GES-1 cells as detected by RT-qPCR. E IL-6 content in HPI GES-1 cells as measured by ELISA. F IL-8 content in HPI GES-1 cells as measured by ELISA. G TNF-α content in HPI GES-1 cells as measured by ELISA. H HP biofilm formation in HPI GES-1 cells as detected by immunofluorescence staining; Scale bar: 50 μm. I Adhesion of HP in HPI GES-1 cells as detected by immunofluorescence staining; Scale bar: 25 μm. Values are expressed as mean ± standard deviation. ***P < 0.001.

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