Fig. 7: Impact of METTL3/NF-κB signaling axis on HPI GES-1 cells. | Cell Death & Disease

Fig. 7: Impact of METTL3/NF-κB signaling axis on HPI GES-1 cells.

From: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

Fig. 7

A Colony formation assay to detect the survival of HPI-treated GES-1 cells. B Flow cytometry analysis to detect apoptosis in HPI-treated GES-1 cells. C Western blot analysis of cleaved caspase-3, cleaved caspase-9, and cleaved PARP levels. D RT-qPCR analysis of IL-6, IL-8, and TNF-α mRNA levels in HPI-treated GES-1 cells. E ELISA analysis of IL-6 levels in HPI-treated GES-1 cells. F ELISA analysis of IL-8 levels in HPI-treated GES-1 cells. G ELISA analysis of TNF-α levels in HPI-treated GES-1 cells. H Immunofluorescence staining to detect biofilm formation in HPI-treated GES-1 cells, scale bar:25 μm; I Immunofluorescence staining to detect HP adhesion in HPI-treated GES-1 cells, scale bar: 25 μm. Values are presented as the mean ± standard deviation, and all cell experiments were repeated three times. *** indicates P < 0.001.

Back to article page