Fig. 8: Effects of METTL3 knockout on HPI gastric inflammation in mice. | Cell Death & Disease

Fig. 8: Effects of METTL3 knockout on HPI gastric inflammation in mice.

From: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

Fig. 8

A Construction of METTL3−/− and CXCL1−/− mice using CRISPR-Pro gene knockout technology, along with RT-qPCR and sequencing results. B Rapid urease test to detect gastric mucosal infection in mice. C H&E staining to observe the morphology and structure of gastric mucosal cells, scale bar: 50 μm. D TUNEL assay to detect apoptosis in gastric mucosal cells, scale bar: 25 μm. E Western blot analysis of cleaved caspase-3, cleaved caspase-9, and cleaved PARP levels. F RT-qPCR analysis of CXCL1, IL-6, IL-8, and TNF-α mRNA levels in the gastric mucosa of mice. G ELISA analysis of CXCL1, IL-6, and TNF-α levels in the gastric mucosa of mice. H Immunofluorescence detection of GIF expression in the gastric mucosa, scale bar: 25 μm. I Immunofluorescence detection of Ki67 expression in the gastric mucosa, scale bar: 25 μm. J Immunofluorescence detection of MPO expression in the gastric mucosa, scale bar: 25 μm. Each group contains 6 mice. Values are presented as the mean ± standard deviation, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

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