Fig. 2: HSF1 knockdown inhibited proliferation, and enhanced apoptosis and sensitivity to vincristine and doxorubicin in DLBCL in vitro. | Cell Death & Disease

Fig. 2: HSF1 knockdown inhibited proliferation, and enhanced apoptosis and sensitivity to vincristine and doxorubicin in DLBCL in vitro.

From: Heat shock factor 1 promotes proliferation and chemoresistance in diffuse large B-cell lymphoma by enhancing the cell cycle and DNA repair

Fig. 2

A Western blotting analysis of HSF1 expression in HSF1-silenced cells and control cells. GAPDH was used as a control. B CCK8 assays of SU-DHL-2, SU-DHL-4 and SU-DHL-6 cells transfected with HSF1 or Ctrl shRNA. Representative images (C) and quantification (D–F) of cell cycle in SU-DHL-2, SU-DHL-4 and SU-DHL-6 cells transfected with HSF1 or Ctrl shRNA were analysed by flow cytometry. IC50 values of vincristine (G) and doxorubicin (H) in SU-DHL-2, SU-DHL-4 and SU-DHL-6 cells with HSF1 knockdown compared with those in the respective control cells. Images (I) and quantification (J) of cell apoptosis in DLBCL cells transfected with control or HSF1 shRNA and treated with vincristine or doxorubicin for 48 h. Statistical significance was evaluated by using a two-tailed t test or one-way ANOVA. The error bars indicate the standard deviations (SDs) of three independent experiments. *p < 0.05, **p < 0.01.

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