Fig. 6: The target genes of HSF1 were identified in DLBCL cells. | Cell Death & Disease

Fig. 6: The target genes of HSF1 were identified in DLBCL cells.

From: Heat shock factor 1 promotes proliferation and chemoresistance in diffuse large B-cell lymphoma by enhancing the cell cycle and DNA repair

Fig. 6

A Volcano plots displaying the gene expression changes in SU-DHL-2 (left) and SU-DHL-6 (right) regulated by DTHIB, compared with DMSO. Differential expression values are plotted against p values; yellow dots represent the upregulated genes, and blue dots represent downregulated genes. B Venn diagram showing the number of downregulated genes in SU-DHL-2 (left) and SU-DHL-6 (right) treated with DTHIB. A total of 677 genes were downregulated in SU-DHL-2, 1210 genes were downregulated in SU-DHL-6, and 340 genes were downregulated in both SU-DHL-2 and SU-DHL-6. C Gene Ontology (GO) analysis identifying the enrichment of biological processes. D Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identifying the enrichment of signaling pathways. Validation of candidate downregulated genes by qRT‒PCR (E) and Western blotting (F) in SU-DHL-2, SU-DHL-4 and SU-DHL-6. GAPDH was used as an internal control. G Chromatin immunoprecipitation (ChIP)-qPCR analysis of negative control IgG and HSF1 enrichment at the promoters of HSF1 target genes in SU-DHL-2 and SU-DHL-6 cells treated with DTHIB or DMSO. The values are normalized to the input values and are presented as the means ± SDs. *p < 0.05, **p < 0.01.

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