Fig. 2: FIBCD1 is upregulated in BC-DM and under hyperglycemia conditions. | Cell Death & Disease

Fig. 2: FIBCD1 is upregulated in BC-DM and under hyperglycemia conditions.

From: Diabetes is causally associated with increased breast cancer mortality by inducing FIBCD1 to activate MCM5-mediated cell cycle arrest via modulating H3K27ac

Fig. 2

A Scatter plotting shows differentially expressed genes between BC-DM tumor tissues and BC-ND tumor tissues. The red dots and green dots represent upregulated and downregulated mRNAs with statistical significance, respectively. B KEGG pathway analysis of significantly up-regulated mRNAs suggests that upregulated genes in BC-DM are enriched in metabolism-related pathways. C KEGG pathway analysis of significantly down-regulated mRNAs. D Gene set enrichment analysis (GSEA) revealed functional enrichments of differentially expressed genes in BC-DM tumor tissues. Normalized enrichment score (NES) and p-values are indicated. E FIBCD1 gene expression levels (detected by qRT-PCR assay) in BC-DM tissues are higher than that in BC-ND tissues. F Representative IHC images showing that FIBCD1 protein expression level in BC-DM tissue is higher than that in BC-ND tissue. G Top upregulated genes in BC-DM tumor tissues with FIBCD1 ranks as the top one DEG. H FIBCD1 gene expression levels in MCF7 cells are glucose dependent. I FIBCD1 protein expression is related to glucose concentration in MCF7 cells. J FIBCD1 gene expression levels in MDA-MB-231 cells under hyperglycemic conditions are higher than in euglycemic conditions. K FIBCD1 protein expression is related to glucose concentration in MDA-MB-231 cells. Data are presented as mean ± SD, Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.

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